Correct dilution first. Then dosing. Then execution.
Use the calculator here: Peptide calculator
What this calculator does (and what it does not)
- It does: convert peptide dose (mcg/mg) into mL / syringe units based on your vial amount and dilution.
- It does not: pick a protocol, decide a dose, or guarantee outcomes.
If you don’t understand the dilution, don’t trust the output.
Before you calculate (pre-flight checklist)
- Confirm the vial amount (e.g. 5 mg, 10 mg) from the label.
- Confirm how much bacteriostatic water you added (e.g. 1 mL, 2 mL).
- Know your target dose in mcg (most peptide protocols are expressed in mcg).
- Pick the syringe scale you’re actually using (typically 1 mL insulin syringe = 100 units).
If you haven’t mixed yet, start here: Peptide reconstitution basics.
The core logic (so the numbers make sense)
Your dilution creates a concentration. The calculator uses that concentration to convert a target dose into a draw volume.
- Concentration: vial amount ÷ added water (e.g. 10 mg ÷ 2 mL = 5 mg/mL)
- Target dose: the amount you want per injection (often mcg)
- Draw volume: target dose ÷ concentration (converted into mL + syringe units)
More water doesn’t give you more peptide. It just spreads the peptide thinner.
Step-by-step: how to enter the inputs
1) Enter vial amount (mg)
- Use the number printed on the vial (commonly 5 mg or 10 mg).
- If the vial label is unclear, stop. Guessing here makes every dose wrong.
2) Enter added water (mL)
- Enter the exact volume of bacteriostatic water added to the vial.
- If you changed dilution mid-vial, you must recalculate. (Better: don’t do that.)
3) Enter your target dose (mcg or mg)
- If your protocol is in mcg, enter mcg.
- If your protocol is in mg, enter mg.
- Do not mix them. 1 mg = 1000 mcg.
4) Choose syringe type / scale
- Most insulin syringes: 1 mL = 100 units.
- Your “units” output is only correct if the syringe scale matches your syringe.
How to read the outputs
- mL to draw = the true liquid volume you need.
- Syringe units = that mL translated onto your syringe markings.
- Concentration = what your dilution produced (useful for sanity checks).
If the syringe units seem extreme (e.g. “2 units” or “80 units”), your inputs are usually wrong: vial amount, water added, or mg vs mcg.
Common mistakes that waste vials
- mg vs mcg confusion (the #1 error)
- Entering the wrong water volume (guessing or rounding)
- Using sterile water instead of bacteriostatic water (shorter usable life, higher risk)
- Poor storage (heat/light/handling degrade potency)
- Changing dilution mid-vial without recalculating
Storage matters more than people admit: Peptide storage & handling
Execution: reduce errors when drawing
- Swab the vial top every time and let it dry.
- Draw slowly and avoid pushing air back into the vial aggressively.
- Use a consistent routine to avoid “misread the syringe” mistakes.
If you want the injection fundamentals: Injection technique & safety overview
Where to go next
- Use the peptide calculator (top “How to use this” link should point here)
- Peptides category overview
- Peptide reconstitution basics
- Peptide storage & handling
Common questions
Can I “choose a dilution” based on what looks easiest on the syringe?
Yes, as long as it’s measured accurately and you stick to it. People get in trouble when they pick a dilution, then guess the mL, or “top it up later” and forget they changed concentration.
Why do people say peptides “stopped working”?
Usually one of these: dilution math error, mg/mcg mistake, degraded peptide from storage/handling, or inconsistent execution. Start by verifying inputs and storage before blaming the compound.
Should I recalc every injection?
Not if your dilution and dose stay the same. Lock your setup, record the syringe units, then execute consistently.
Key takeaways
- Correct dilution is the foundation. Everything else depends on it.
- Most peptide errors are unit errors (mg vs mcg) or wrong water volume.
- Match the calculator to your syringe scale or the “units” output is meaningless.
- Storage and handling can quietly destroy potency even if the liquid looks normal.